Molecular-Weight Analysis of Helix pomatia alpha-Hemocyanin in Guanidine Hydrochloride, Urea and Sodium Dodecylsulfate
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چکیده
منابع مشابه
Molecular weight and hydrodynamic properties of apolipoprotein B in guanidine hydrochloride and sodium dodecyl sulfate solutions.
The apolipoprotein B polypeptide of human serum low density lipoprotein exists (after reduction of disulfide bonds) as a random coil with a molecular weight of 250,000 in concentrated solutions of guanidine hydrochloride. With intact disulfide bonds, there is a limited restraint on the polypeptide conformation in this denaturing solvent. In the presence of saturating amounts of bound sodium dod...
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The small-angle X-ray scattering of 1/10-size molecules (in 0.1 ionic strength Tris buffer pH 8.1) and 1/20-size molecules (in 0.1 ionic strength ethanolamine buffer pH 10.6) of a-hemocyanin of the snail Helix pomatia was studied. The radii of gyration, 8.7 nm and 8.0 nm for 1/10-size and 1/20-size molecules respectively, and the shape of the scattering curves indicate that both have a very ani...
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Hemocyanin of the snail helix pomatia was studied by small angle X-ray scattering in 0.15 M phosphate buffer pH 6. The outer portion of the scattering curve showed minima, which indicated that the hemocyanin molecule is composed of a large number of identical (or very similar) nearly spherical subunits. From the position of the minima the radius of gyration R u of the subunit was calculated to ...
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The molecular weight of Escherichia coli beta-galactosidase was determined in 6m- and 8m-guanidine hydrochloride by meniscus-depletion sedimentation equilibrium, sedimentation velocity and viscosity. Sedimentation equilibrium revealed heterogeneity with the smallest component having a molecular weight of about 50000. At lower speeds, the apparent weight-average molecular weight is about 80000. ...
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the west of esfahan province, iran, is one of the most important agricultural areas throughout the country due to the climate variability and life-giving water of zayanderood river. rice is one of the major and economic crops in this area. the most important climatic elements in agricultural activities which should be considered include temperature, relative humidity, precipitation and wind. so...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1973
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1973.tb02855.x